The Effect of Sambiloto Extract on the Expression of β Estrogen Receptors in Ovaries and Lust Cycle in Mice Model PCOS - Insulin Resistance


  • Hanis Kusumawati Rahayu Universitas Airlangga



PCOS, Insulin Resistance, Sambiloto, Estrus Cycle, Expression Of Estrogen Receptor β


Infertility was a serious problem in the reproductive period, one of the caused of infertility were common in women of childbearing age group is Polycystic Ovary Syndrome (PCOS).Sambiloto (Andrographis paniculata Nees), had been already known contain flavonoid and lactones. The purpose of this research had to determine the effect of various doses of sambiloto extract with the expression of β estrogen receptor and estrus cycle in the mice model of PCOS - insulin resistance. This research was an animal model experimental laboratory research with a completely randomized design (CRD).The results showed that sambiloto extract therapy in treatment group had an improvement estrus cycle compared with control group. Positive control group, almost 90% had persistent anestrus condition. In treatment group 1 and 2 in the last vagina smear obtained anestrus condition as much as 60% in diestrus and medestrus phase. Whereas treatment groups 3 all experimental animals in a estrus state by proestrus and estrus phase. β Estrogen receptor expression had tested by Kruskal Wallis test for overall treatment, obtained significantly different results (p ≤ 0.002) followed by Mann - Whitney, showed that immunoreactive cells score from highest to lowest occurred in each group Negative control (KN), P3, P2, P1 and Positive Control (KP).

It could be concluded that sambiloto extract at dose 18mg/kgbb, 36mg/kgbb, and 72mg/kgbb had been given an overview of the differences expression of β estrogen receptor, could change the estrus cycle of female mice models of insulin resistance PCOS.

Author Biography

Hanis Kusumawati Rahayu, Universitas Airlangga

Ilmu Kesehatan Reproduksi


Bjornstrom L & Sjoberg M, 2005. Mechanism of Estrogen Receptor Signaling: Convergence of Genomic and non Genomic Actions on Target Genes. Molecular Endocrinology 3: 1-24.

Dunaif A. 1997. ‘Insulin Resistance And Polycystic Ovary Syndrome: Mechanism And Implication For Pathogenesis’. JournalEndocrine Reviews vol 18 no 6 : 744-810

Harris HA, 2007. Estrogen Receptor B: Resent Lessons from Invivo Studies. Molecular Endocrinology 21(1):1-13

Hopkinson ZE, Sattar N, and Fleming R. 1998. ‘Polycystic ovarian syndrome: the metabolic syndrome comes to gynaecology’.BMJ;317: 329-33.

Kasdu, D. 2002. ‘Kiat Sukses Pasangan Memperoleh Keturunan’. Jakarta : Pustaka Pembangunan Swadaya Nusantara

Kato S, Masuhiro Y, Watanabe M, Kobayashi Y, Takeyama K, Endok H, Yanagisawa J, 2000. Molecular Mechanis of a Cross-talk between Estrogen and Growth Factor Signaling Pathways. Genes to cell 5: 593-601

Kuiper GGJM, Lemmen JG, Carlsson B, Corton CJ, Safe SH, Gustafsson J, 1998. Interaction of Estrogenic Chemicals and Phytoestrgens with ER-β, J. Endrocrinology vol. 139, No. 10: 4252-63

Legro RS. 2001. ‘Diabetes prevalence and risk factors in polycystic ovary syndrome’.Obstetrics and Gynecology Clinics of North America; 28: 99-109

Muttaqin, Dwinanto Ananda, Budi Santoso, Wdjiati, 2008. Pengaruh Lama Paparan Androgen terhadap Indeks Resistensi Insulin dan Kadar Asam Lemak Bebas pada Serum Tikus Model SOPK. Fakultas Kedokteran Universitas Airlangga

Pedram A, Razandi, Aid kenhead M, Hughes CCW, Levin ER, 2002. Integmiceion of the Non Genomic and Genomic Actions of Estrogen. J. Biol. Chem. 277(52):50768-50775

Prapanza, E. Dan Marianto, L.M. 2003. ‘Khasiat & Manfaat Sambiloto: Raja Pahit Penakluk Aneka Penyakit’. AgroMedia Pustaka. Hal: 3–9.

Sheehan, MT. 2004. ‘Polycystic Ovarian Syndrome: Diagnosis And Management. Clinical Medicine & Research’. 2(1):13–27.

Razandi M, Pedram A, Greene GL, Levin ER, 1999. Cell Membrane and Nuclear Estrogen Receptor (ERs) Originate from a Single Transcript: Studies ER α and ER β expressed in CHO cell. Molecular Endocrinology 13: 307-319

Tapan, E. 2003. ‘Simposium Sehat dan Cantik Pra dan Pasca-Menipause Bersama Calvonin. Available online at

Wang C Y, Zhang ZT, Shen P, Loggie BWChang YCDeuel TF, 2006. A Varian of Estrogen Receptor hER36; Tranduction of Estrogen and Antiestrogen-Dependent MembranInitiated Mitogenic Signaling. PNAS 103(24): 9063-9068.

Winarto WP. 2004. ‘Sambiloto: Budidaya Dan Pemanfaatan Untuk Obat’. Penebar Swadaya, Jakarta Hal 1-12

Yulinah, E., Sukrasno.,dan Fitri, A. 2001. ‘Aktivitas Ekstrak Etanol Herba Sambiloto (Andrographis paniculata Ness)’. Jurusan Farmasi FMIPA, ITB